Creatine is among the most popular and extensively studied sports nutrition supplements, recognized for its proven ability to enhance strength, muscle mass, and performance in high-intensity, short-duration activities. Accurate determination of creatine and its degradation product, creatinine, in raw materials and finished products is essential for quality control and regulatory compliance. 

This webinar will provide an overview of best practices in HPLC creatine and creatinine testing in diverse range of products, including protein powders, pre-workout formulations, beverages, and gummies. Common analytical challenges and crucial factors affecting the accuracy of the reported results, including creatine form, sample size, solubility, and analyte stability in standards and sample extracts, will be discussed. Performance of the internal Eurofins Food Chemistry Testing Madison, Inc. method will be assessed against AOAC International acceptance criteria.

Highlights

• Gain insight into creatine and creatinine HPLC testing workflow. 

• Learn how to address common analytical challenges, avoid pitfalls and ensure obtaining accurate results representative of the test material.

• Understand the importance of a thorough validation process and acceptance criteria setup to ensure the creatine test method is scientifically valid and fit-for-purpose. 

Meet the Presenter

Dr. Lukas Vaclavik is a Technical Manager at Eurofins Food Chemistry Testing Madison, Inc, specializing in contaminant and residue testing. In his current role, he leads initiatives to ensure the safety and quality of food products through advanced chemical analysis. Before joining Eurofins, he held a position at the Center for Food Safety and Applied Nutrition (CFSAN) at the U.S. Food and Drug Administration (FDA), where he contributed to regulatory science and research. 

With a strong academic and professional background in chromatographic and mass spectrometric techniques, Dr. Vaclavik has authored or co-authored more than 40 peer-reviewed scientific publications. His research focuses on the detection and analysis of chemical residues, contaminants, and adulterants in food and related products. 

Dr. Vaclavik holds a Ph.D. in Food Chemistry and Analysis from the University of Chemistry and Technology in Prague, Czech Republic. 

Copyright © 2025 Eurofins Food Chemistry Testing Des Moines